期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 276, 期 18, 页码 14946-14954出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M010911200
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The Streptococcus pneumoniae mal regulon contains two operons, malXCD and malMP involved in the uptake and utilization of maltosaccharides. Both operons are transcribed from two divergent promoters, P-X and P-M, and are negatively regulated by the MalR transcriptional repressor. Purified MalR protein binds to two DNA regions that encompasses both promoters, thus occupying its two operators, O-M and O-X. However, the levels of occupation and repression were different, being higher when MalR was bound to O-M than when it was anchored to O-X. Competition experiments between MalR and the Escherichia coli RNA polymerase on promoters P-M and P-X showed that the affinity of either protein for the promoter/operator DNA sequences was important to determine the frequency of transcription initiation. In addition to the control exerted by MalR, expression from promoter P-M was affected by upstream sequences located within or close to P-X promoter.
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