期刊
BIOCHEMICAL JOURNAL
卷 425, 期 -, 页码 207-214出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20091290
关键词
[4Fe-4S]-cluster-containing P-loop NTPase (FSC-NTPase); Arabidopsis thaliana; chloroplast; high chlorophyll fluorescence 101 (HCF101); iron-sulfur cluster assembly; scaffold protein
资金
- Deutsche Forschungsgemeinschaft [SFB 593, TR1, GRK 1216, ME 1794/4]
- Gottfried-Wilhelm Leibniz programme
- Fonds der chemischen Industrie
Oxygen-evolving chloroplasts possess their own iron-sulfur cluster assembly proteins including members of the SUF (Sulfur mobilization) and the NFU family. Recently, the chloroplast protein HCF101 (high chlorophyll fluorescence 101) has been shown to be essential for the accumulation of the membrane complex Photosystem I and the soluble ferredoxin-thioredoxin reductases, both containing [4Fe-4S.] clusters. The protein belongs to the FSC-NTPase ([4Fe-4S]-cluster-containing P-loop NTPase) superfamily, several members of which play a crucial role in Fe/S cluster biosynthesis. Although the C-terminal ISC-binding site, conserved in other members of the FSC-NTPase family, is not present in chloroplast HCF101 homologues using Mossbauer and EPR spectroscopy, we provide evidence that HCF101 binds a [4Fe-4S] cluster. (55)Fe incorporation studies of mitochondrially targeted HCF101 in Saccharomyces cerevisiae confirmed the assembly of all Fe/S cluster in HCF101 in an Nfs1-dependent manner. Site-directed mutagenesis identified three HCF101-specific cysteine residues required for assembly and/or stability of the cluster. We further demonstrate that the reconstituted cluster is transiently bound and can be transferred from HCF101 to a [4Fe-4S] apoprotein. Together, our findings suggest that HCF101 may serve Lis a chloroplast scaffold protein that specifically assembles [4Fe-4S] clusters and transfers them to the chloroplast membrane and soluble target proteins.
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