期刊
JOURNAL OF CHROMATOGRAPHY B
卷 755, 期 1-2, 页码 297-303出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00125-6
关键词
di(2-ethylhexyl) phthalate
A simple, rapid and sensitive reversed-phase high-performance liquid chromatographic method with UV detection was developed for the quantification of di(2-ethylhexyl) phthalate (DEHP) in parenteral nutrition admixtures containing fat emulsion and in plasma samples of children daily treated by total parenteral nutrition. The analyte and the internal standard, di-n-heptyl phthalate, were extracted twice using hexane and the organic layer separated and dried under nitrogen. The residues were reconstituted with acetonitrile and 20 mul was injected into a Waters Spherisorb C-18 column, the UV detector was set at 202 nm. The mobile phase was acetonitrile-aqueous buffer (triethylamine 0.08% adjusted to pH 2.8 with 1 M phosphoric acid) mixture (88:12, v/v) and it was pumped at 1 ml/min. Average recoveries were 97% or greater. This method was successfully used to investigate the amounts of DEHP which can leach from bags and tubing into fat emulsion and which could contaminate children under long-term parenteral nutrition. On the other hand, the circulating DEHP concentrations were estimated in four children under regular long-term, parenteral nutrition. (C) 2001 Elsevier Science B.V. All rights reserved.
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