期刊
BIOCHEMICAL JOURNAL
卷 430, 期 -, 页码 215-221出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20100340
关键词
DT40 cell; genomic tagging; nuclear signalling; phosphatidylinositol; phosphatidylinositol 5-phosphate kinase
资金
- Wellcome Trust [079194/Z/06]
- Engineering and Physical Sciences Research Council [EP/D013615/1]
- Biotechnology and Biological Sciences Research Council [BB/F011067/1] Funding Source: researchfish
- BBSRC [BB/F011067/1] Funding Source: UKRI
PtdIns5P 4-kinases II alpha and II beta are cytosolic and nuclear respectively when transfected into cells, including DT40 cells [Richardson, Wang, Clarke, Patel and Irvine (2007) Cell. Signalling 119, 1309-1314]. In the present study we have genomically tagged both type II PtdIns5P 4-kinase isoforms in DT40 cells. Immunoprecipitation of either isoform from tagged cells, followed by MS, revealed that they are associated directly with each other, probably by heterodimerization. We quantified the cellular levels of the type H PtdIns5P 4-kinase mRNAs by real-time quantitative PCR and the absolute amount of each isoform in immunoprecipitates by MS using selective reaction monitoring with N-14,C-13-labelled internal standard peptides. The results suggest that the dimerization is complete and random, governed solely by the relative concentrations of the two isoforms. Whereas PtdIns5P 4-kinase II beta is >95% nuclear, as expected, the distribution of PtdIns4P 4-kinase II alpha is 60% cytoplasmic (all bound to membranes) and 40% nuclear. In vitro, PtdIns5P 4-kinase IIa was 2000-fold more active as a PtdIns5P 4-kinase than the II beta isoform. Overall the results suggest a function of PtdIns5P 4-kinaseII beta may be to target the more active ha isoform into the nucleus.
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