期刊
BIOCHEMICAL JOURNAL
卷 428, 期 -, 页码 483-489出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20100143
关键词
calcium channel; electron microscopy (EM); inositol 1,4,5-trisphosphate receptor (IP3R); single-particle analysis (SPA)
资金
- Wellcome Trust [085295]
- Institute of Cancer Research
- Newton Trust, Cambridge
IP(3)Rs (inositol 1,4,5-trisphosphate receptors) are the intracellular channels that mediate release of Ca2+ from the endoplasmic reticulum in response to the many stimuli that evoke Ins(1,4,5)P-3 formation. We characterized and purified type 1 IP3R heterologously expressed in Sf9 insect cells, and used the purified IP(3)R1 to determine its three-dimensional structure by electron microscopy and single-particle analysis. Recombinant IP(3)R1 has 4-fold symmetry with overall dimensions of approx. 19.5 nm x 19.5 nm x 17.5 nm. It comprises a small domain, which is likely to include the pore, linked by slender bridges to a large cytoplasmic domain with four petal-like regions. Our structures of recombinant IP(3)R1 and native cerebellar IP3R have similar appearances and dimensions. The only notable difference is the absence of a central stigma-like domain from the cytoplasmic region of recombinant IP(3)R1. The first structure of a recombinant IP3R is an important step towards developing three-dimensional structures of IP3R that better contribute to our understanding of the structural basis of IP3R activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据