期刊
BIOCHEMICAL JOURNAL
卷 421, 期 -, 页码 51-58出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20090242
关键词
hydrogen peroxide; mitochondria; peroxiredoxin; oxidative stress; sulfenic acid; thiol
资金
- Health Research Council of New Zealand
- National Research Centre for Growth and Development
- National Institutes of Health [R01GM0728661]
- Tertiary Education Commission of New Zealand
The Prxs (peroxiredoxins) are a family of cysteine-dependent peroxidases that decompose hydrogen peroxide. Prxs become hyperoxidized when a sulfenic acid formed during the catalytic cycle reacts with hydrogen peroxide. In the present study, Western blot methodology was developed to quantify hyperoxidation of individual 2-Cys Prxs in cells. It revealed that Prx 1 and 2 were hyperoxidized at lower doses of hydrogen peroxide than would be predicted from in vitro data, suggesting intracellular factors that promote hyperoxidation. In contrast, mitochondrial Prx 3 was considerably more resistant to hyperoxidation. The concentration of Prx 3 was estimated at 125 mu M in the mitochondrial matrix of Jurkat T-lymphoma cells. Although the local cellular environment could influence susceptibility, purified Prx 3 was also more resistant to hyperoxidation, suggesting that despite having C-terminal motifs similar to sensitive eukaryote Prxs, other structural features must contribute to the innate resilience of Prx 3 to hyperoxidation.
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