期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 193, 期 10, 页码 1199-1212出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.193.10.1199
关键词
chemokine; pregnancy; leukocyte; immune tolerance; trophoblast
资金
- NHLBI NIH HHS [HL64597, R01 HL064597] Funding Source: Medline
During human pregnancy, the specialized epithelial cells of the placenta (cytotrophoblasts) come into direct contact with immune cells in several locations. In the fetal compartment of the placenta, cytotrophoblast stem cells lie adjacent to macrophages (Hofbauer cells) that reside within the chorionic villus stroma, At sites of placental attachment to the mother, invasive cytotrophoblasts encounter specialized maternal natural killer (NK) cells (CD56bright), macrophages, and T cells that accumulate within the uterine wall during pregnancy. Hare we tested the hypothesis that fetal cytotrophoblasts carl direct the migration of these maternal immune cells. First, we assayed the chemotactic activity of cytotrophoblast conditioned medium samples, using human peripheral blood mononuclear cells as targets. The placental samples preferentially attracted NK cells (both CD56(dim) and CD56(bright)), monocytes. and T cells, suggesting that our hypothesis was correct. A screen to idrntifv chemokine activity through the induction of a Ca transfected with individual chemokine receptors suggested that cytotrophoblasts secreted monocyte inflammatory protein (MIP)-1 alpha. This was confirmed by localizing the corresponding mRNA and protein, both in vitro and in vivo. MIP-1 alpha protein in conditioned medium was further characterized by immunoblotting and enzyme-linked immunosorbent assay. Immunodepletion of MIP-1 alpha from cytotrophoblast conditioned medium showed that this chemokine was responsible for a significant portion of the induced monocyte and CD56(bright) NK cell chemotaxis. These data suggest the specific conclusion that cytotrophoblasts carl attract monocytes and CD56(bright) NK cells by producing MIP-1 alpha and the more general hypothesis that these cells may organize and act on leukocytes at the maternal-fetal interface.
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