期刊
NEUROSCIENCE LETTERS
卷 304, 期 3, 页码 189-193出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(01)01769-4
关键词
three-dimensional culture; cell immobilization; extracellular matrix; collagen; agarose; neurons; apoptosis; tissue engineering
To better understand interactions between neurons and extracellular matrix equivalents, embryonic day-18 rat cortical neurons were immobilized and maintained in culture for up to 24 days in agarose and type I collagen gels. Using live/ dead staining, neuronal cultures in low density collagen gel lasted at least 3 weeks. At 14 days, over 50% of immobilized cells in collagen gel were found viable while in low density agarose gel no cells survived. In situ cell death detection showed that most, if not all, dead cells in either of the gels underwent apoptosis. The collagen-trapped neurons exhibited normal neuronal polarity and developed long neurites, estimated at over 500 mum. The results suggest that collagen, because it is a major extracellular matrix constituent, suppresses apoptosis and provides a suitable substrate for neuronal survival and differentiation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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