Pattern of protein production by mammary epithelial cells cultured on membrane inserts

Protein and plasminogen activator (PA) production by bovine mammary epithelial cells cultured on inserts and the effect of calcium were studied. The cells cultured on inserts coated with a bovine extracellular matrix established a stable trans-epithelial resistance, and secreted apically alpha (s1)-casein and PA. Extracellular calcium increased (P < 0.01) alpha (s1)-casein and total proteins, but not PA activity.