期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 88, 期 -, 页码 131-141出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2014.04.009
关键词
Immobilization; Lipase; Adsorption; Bioconversion; Mesoporous silica spheres; Polyethyleneimine
资金
- Biotechnology Research Center, Tehran University of Medical Sciences [91-03-90-19634]
- Iran National Science Foundation (INSF), Tehran, Iran [92009042]
In the present study, silicate mesoporous materials (MCM-41), MCM-41-grafted polyethylenimine (MCM-41@PEI), and succinated PEI containing amine, amide, and acid groups were successfully synthesized and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Brunauer-Emmett-Teller analysis, and X-ray photoelectron spectroscopy. Thermomyces lanuginosa lipase (TLL) was then immobilized onto MCM-41 and polymer-grafted MCM-41 by physical adsorption. Besides, for enzyme immobilization via covalent bonding, glutaraldehyde (GLU), and hexamethylene diisocyanate (HMDI) were used as the bridges for binding the enzyme to supports. The best result was obtained with the immobilized lipase on MCM-41@PEI-GLU. In the study of the enzyme reusability, it was shown that about 83% of the initial activity could be retained after 12 cycles of uses. The immobilized lipase on the selected support was also applied for the synthesis of ethyl valerate. Following 24 h incubation in n-hexane and solvent free media, the esterification percentages were 79% and 67%, respectively. (C) 2014 Elsevier B.V. All rights reserved.
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