期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 74, 期 -, 页码 15-19出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bej.2013.02.008
关键词
Solid-state fermentation; Alkaline protease; Respiration activity; Experimental design; Bench scale production
资金
- Spanish Ministerio de Economia y Competitividad [CTM2012-33663]
- UAB
The production of alkaline proteases by solid-state fermentation (SSF) was evaluated. The effect of three agro-industrial residues was examined: coffee husk, hair waste from the tanning industry and soy fibre residues. Soy fibre presented the highest yield for protease production at the laboratory scale (37 degrees C, 100 g samples). Consequently, experiments with soy fibre (F) and soy fibre with 10% compost as an inoculum (FC) were performed for 14 days in 4.5 L bench-scale aerobic near-adiabatic reactors. The highest activity occurred under thermophilic conditions and a high respiration activity of 47,331 +/- 1391 U/g dry matter for F and 18,750 +/- 1596 U/g dry matter for FC, which are much higher values than those reported in other studies. Alkaline proteases showed maximum stability at pH 11 and temperatures of 43.8 degrees C (F) and 30 degrees C (FC), which was determined by a full factorial experimental design. Storage assays demonstrated that 90% of enzyme activity was preserved for three months by lyophilising or freezing the samples at -80 degrees C. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据