期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 74, 期 -, 页码 1-7出版社
ELSEVIER
DOI: 10.1016/j.bej.2013.02.005
关键词
Haloalcohol dehalogenase; Epoxide hydrolase; Two-phase system; (R)-Epichlorohydrin; Immobilized cells
资金
- National Basic Research Program of China (973 Program) [2011CB710806]
- National Natural Science Foundation of China [21176224]
- Key Project for Natural Science Foundation of Zhejiang Province [Z4080032]
Recombinant Escherichia coli cells harbouring haloalcohol dehalogenase and epoxide hydrolase were successfully immobilized by adsorption onto perlite and used to prepare (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis in a specially designed reactor. Two-phase solution was used as the reaction system in order to improve the yield of epichlorohydrin. In the two-phase system containing 40% (v/v) cyclohexane, the yield of racemic epichlorohydrin formed in the first step was 73%, and the yield of (R)-epichlorohydrin with enantiomeric excess (ee) >= 99% increased from 19.2% to 25.1% in the second step. Ultimately, the yield of (R)-epichlorohydrin reached 26.4% by optimization of the flow rate of air and amount of immobilized cells. To our knowledge, this was the first report on production of (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis using halo alcohol dehalogenase and epoxide hydrolase. (C) 2013 Elsevier B.V. All rights reserved.
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