期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 46, 期 1, 页码 28-33出版社
ELSEVIER
DOI: 10.1016/j.bej.2009.04.008
关键词
Bacillus subtilis; Metabolic capacity; Purf gene; Purine pathway; Riboflavin production; Co-overexpression
资金
- National Natural Science Foundation of China [NSFC-20536040]
- National Project of Key Fundamental Research [2007CB707802]
- Development Project of Science and Technology of Tianjin [05YFGZGX04500]
Purine nucleotides are precursors for riboflavin production, and one of its intermediates, GTP, is the immediate precursor for riboflavin biosynthesis. Therefore, activation of the purine pathway would improve the supply of the precursors, which may in turn lead to an increase of riboflavin production. Here we reported the characterization and manipulation of the purine pathway in a riboflavin overproducer Bacillus subtilis PK. Due to the tight regulation of the purine pathway, it was not sufficient to obtain an enhanced production of purine nucleotides or riboflavin by simple overexpression of the purF gene, which is the key gene of the purine pathway. However, co-overexpression of purF, purM, purN, purH, purD genes gave an engineered strain B. subtilis PK-P with much better performance. Metabolites analysis revealed that the precursors, purine nucleotides, were significantly improved in B. subtilis PK-P. Moreover, as much as a 25% increase in the riboflavin yield and a 31% increase in riboflavin titer were obtained in the recombinant B. subtilis PK-P. It was assumed that the co-overexpression of the genes caused higher supply of the purine nucleotides in B. subtilis PK-P, which in turn facilitated riboflavin production. (C) 2009 Elsevier B. V. All rights reserved.
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