Optimization of synergistic parameters for thermostable cellulase activity of Aspergillus heteromorphus using response surface methodology

This Study Was undertaken to find Out the optimum reaction conditions, i e pH. temperature, enzyme and substrate concentration for the thermostable cellulase enzyme activity of Aspergillus heteromorphus using response Surface methodology (RSM) Estimated optimum conditions for cellulase enzyme were found as pH 4 8. temperature 60 degrees C, crude enzyme/substrate concentration ratio was 4 6 The maximum enzymatic activity calculated was observed 13 05 U/ml against the predicted value 11 26 U/ml Cellulase system of A heteromorphus is thermostable retaining 600% of residual activity after I h of pre-incubation at 901,C when assay is carried out at same temperature. Value of correlation coefficient (R-2 0 9977) and significant Value for model p < 00001 indicated validity of model fitness and adequate for optimization (C) 2009 Elsevier B V. All rights reserved.