期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 45, 期 3, 页码 226-231出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bej.2009.04.002
关键词
Tissue engineering; Culture medium; Bioartificial organs; Animal cell culture engineering; Cell engineering; Collagen gel
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18360399]
- Grants-in-Aid for Scientific Research [18360399] Funding Source: KAKEN
Co-culture of primary rat hepatocytes with hepatic non-parenchymal cells or sinusoidal endothelial cells for albumin production activity as an index of liver-specific function was studied. The co-cultures were effective for the expression and maintenance of albumin production activity. However, the co-culture effect was not observed when we used a suitable culture medium, which had already been reported to be sufficient for albumin production activity. Albumin production of dispersed cells in collagen get culture was higher than that of spheroid culture. In addition, albumin production of spheroids in collagen gel culture was higher than that of spheroid culture and dispersed cell collagen gel culture with a suitable culture medium. We found that culture medium composition was more important than co-culture for expression and maintenance of albumin production. Furthermore, we found that cell-cell interaction was effective for the expression of albumin production, but heterotypic cell-cell interaction was not necessary. (C) 2009 Elsevier B.V. All rights reserved.
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