期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 41, 期 1, 页码 24-29出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bej.2008.03.002
关键词
nonionic surfactant; cloud point system; liquid-liquid extraction; biorransformations; microemulsions; downstream processing
A downstream process strategy for a whole microbial transformation to produce L-phenylacetylcarbinol (PAC) in a nonionic surfactant Triton X mediated cloud point system was developed. By application of a Winsor I microemulsion, the product and the nonionic surfactant in the microbial transformation broth was separated successfully. Then the nonionic surfactant was recovered with a Winsor 11 microemulsion. In a single stage Winsor I microemulsion extraction process, the product recovery ratio 76.9% and the nonionic surfactant recovery ratio 66.5% were achieved. A discrete countercurrent extraction operation was also carried out to improve the separation efficiency. Finally, character of the recovery nonionic SUrfaCtant was also examined. (c) 2008 Elsevier B.V. All rights reserved.
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