期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 42, 期 3, 页码 217-223出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2008.06.018
关键词
Membrane bioreactors; Proteolysis; Enzyme bioreactors; Optimisation; Whey protein hydrolysates; Enzyme reuse
资金
- Spanish Plan Nacional I+D+I [PPQ-2002-02235, CTQ-2005-02653/PPQ]
A cyclic batch enzyme membrane reactor (CBEMR) incorporating a 8000-Da polyethersulphone membrane was intended for enhancing the enzyme (Protex 6L from Bacillus licheniformis, EC.3.4.21.62) use in the production of a whey protein hydrolysate. A mechanistic mathematical model comprising zero-order kinetics for the hydrolysis and second-order deactivation for the enzyme was proposed and validated through experiments. The influence of reaction temperature was studied and process optimisation (given the production requirements) was performed in terms of number of batch reactions that minimise the total amount of enzyme used. The optimal operation of the CBEMR allowed savings of up to 44 and 32% of enzyme compared to the single batch operation mode at 50 and 60 degrees C, respectively. No enzyme savings were detected when temperature was fixed at 70 degrees C. In general, the optimal operation temperature was 60 degrees C, yielding lower enzyme consumption for all productivities of the reactor. (C) 2008 Elsevier B.V. All rights reserved.
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