期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 82, 期 1-3, 页码 247-257出版社
HUMANA PRESS INC
DOI: 10.1385/BTER:82:1-3:247
关键词
selenium; tRNA; selenouridine; germinating barley
Selenium-containing tRNA was discovered in germinating barley for the first time with the Se-75 isotopic tracer technique; therefore, this technique was used to study the effect of different concentrations of selenium and sulfur in the medium on the content of selenium-containing tRNA in germinating barley. Se-containing tRNAs and its hydrolysates were isolated, purified, and characterized by means of column chromatography, ion-exchange chromatography, high-performance liquid chromatography, and the ultraviolet-visible spectrum. The results show that the amount of selenium in tRNA is almost unaffected by the sulfuric content in the medium, and the pathway for selenium and sulfur to enter tRNA might not be exactly the same. Selenium exists within tRNA in the form of 5-methylamine methyl-2-selenouridine, just as it does within a microorganism tRNA.
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