期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 94, 期 3, 页码 213-223出版社
HUMANA PRESS INC
DOI: 10.1385/ABAB:94:3:213
关键词
2-keto-L-gulonic acid; Gluconobacter oxydans; glucose dehydrogenase; 2,5-diketo-D-gluconic acid reductase; 2,5-diketo-D-gluconic acid; L-ascorbic acid; glucose; gluconic acid; NADP(H) regeneration
Substrate selectivity of Gluconobacter oxydans (ATCC 9937) for 2,5-diketo-D-gluconic acid (2,5-DKG) production was investigated with glucose, gluconic acid, and gluconolactone in different concentrations using a resting-cell system. The results show that gluconic acid was utilized favorably by G. oxydans as substrate to produce 2,5-DKG. The strain was coupled with glucose dehydrogenase (GDH) and 2,5-DKG reductase for synthesis of 2-keto-L-gulonic acid (2-KLG), a direct precursor Of L-ascorbic acid, from glucose. NADP and NADPH were regenerated between GDH and 2,5-DKG reductase. The mole yield of 2-KLG of this multienzyme system was 16.8%. There are three advantages for using the resting cells of G. oxydans to connect GDH with 2,5-DKG reductase for production of 2-KLG: gluconate produced by GDH may immediately be transformed into 2,5-DKG so that a series of problems generally caused by the accumulation of gluconate would be avoided; 2,5-DKG is supplied directly and continuously for 2,5-DKG reductase, so it is unnecessary to take special measures to deal with this unstable substrate as it was in Sonoyama's tandem fermentation process; and NADP(H) was regenerated within the system without any other components or systems.
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