期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 445, 期 4, 页码 694-701出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.12.070
关键词
Epithelial ovarian cancer; Exosomes Mass spectrometry; Proteomics; Trifluoroethanol
资金
- Canadian Institute of Health Research [MOP-93772]
- Ontario Ministry of Health and Long Term Care
- Canadian Research Chairs Program
- Medical Biophysics Excellence Award
- Kristi Piia Callum Memorial Fellowship
Molecular communication between cancer cells and its stromal microenvironment is a key factor for cancer progression. Alongside classic secretory pathways, it has recently been proposed that small membranous vesicles are alternative mediators of intercellular communication. Exosomes carry an effector-rich proteome with the ability to modulate various functional properties of the recipient cell. In this study, exosomes isolated from four epithelial ovarian cancer cell lines (OVCAR3, OVCAR433, OVCAR5 and SKOV3) were characterized using mass spectrometry-based proteomics. Using an optimized workflow consisting of efficient exosome solubilization and the latest generation of proteomic instrumentation, we demonstrate improved detection depth. Systematic comparison of our cancer cell line exosome proteome against public data (Exocarta) and the recently published NCI 60 proteome revealed enrichment of functional categories related to signaling biology and biomarker discovery. (C) 2014 Elsevier Inc. All rights reserved.
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