期刊
NEUROBIOLOGY OF DISEASE
卷 8, 期 3, 页码 469-478出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/nbdi.2001.0382
关键词
Alzheimer's disease; calcium; confocal microscopy; endoplasmic reticulum; phosphoinositide; presenilin; Xenopus oocytes
资金
- NIA NIH HHS [AG16573, AG15409, AG00096-17] Funding Source: Medline
- NIGMS NIH HHS [GM 48071] Funding Source: Medline
Mutations in presenilin-1 (PS1), the leading cause of early-onset, autosomal-dominant familial Alzheimer's disease (FAD), enhance calcium signaling mediated by inositol 1,4,5-trisphosphate (IP3). To elucidate the subcellular mechanisms underlying this enhancement, we used high resolution line-scanning confocal microscopy to image elementary calcium release events (puffs) in Xenopus oocytes expressing wild-type or mutant PS1. Here we report that mutant pal-rendered puffs more sensitive to IP3 and increased both the magnitude and the rate of calcium release during each event. These effects were not attributable to quantitative changes in the levels of IP3 receptors or their distribution on the ER, but were instead associated with an abnormal elevation of ER calcium stores. Together, our results suggest that the effects of mutant pal on calcium signaling are manifested predominantly at the level of the regulation of calcium stores rather than via perturbations in the numbers or activity of IP3-activated calcium release channels. (C) 2001 Academic Press.
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