期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 450, 期 4, 页码 1439-1445出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.07.011
关键词
Tomato; DDI1; DCAF protein; CUL4-DDB1; Abiotic stress
资金
- National Science Fund for Distinguished Young Scholars [30825030]
- National Natural Science Foundation of China [31171179, 90717110]
- 973 Program of China [2011CB100401]
- Ministry of Education of China [20110181130009]
CULLIN4(CUL4)-DAMAGED DNA BINDING PROTEIN1 (DDB1)-based ubiquitin ligase plays significant roles in multiple physiological processes via ubiquitination-mediated degradation of relevant target proteins. The DDB1-CUL4-associated factor (DCAF) acts as substrate receptor in the CUL4-DDB1 ubiquitin ligase complex and determines substrate specificity. In this study, we identified a tomato (Solanum lyco-persicum) DDB1-interacting (DDI1) protein as a DCAF protein involved in response to abiotic stresses, including UV radiation, high salinity and osmotic stress. Co-immunoprecipitation and bimolecular fluorescence complementation assay indicated that DDI1 associates with CUL4-DDB1 in the nucleus. Quantitative RT-PCR analysis indicated the DDI1 gene is induced by salt, mannitol and UV-C treatment. Moreover, transgenic tomato plants with overexpression or knockdown of the DDI1 gene exhibited enhanced or attenuated tolerance to salt/mannitol/UV-C, respectively. Thus, our data suggest that DDI1 functions as a substrate receptor of the CUL4-DDB1 ubiquitin ligase, positively regulating abiotic stress response in tomato. (C) 2014 Elsevier Inc. All rights reserved.
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