期刊
TOXICON
卷 39, 期 6, 页码 831-836出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0041-0101(00)00215-4
关键词
blue-green algae; cyanobacteria; toxins; microcystins; time-resolved fluoroimmunoassay
An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1 mug/l. The derection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time. (C) 2001 Elsevier Science Ltd. All rights reserved.
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