期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 452, 期 3, 页码 593-599出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2014.08.118
关键词
Carbon nanotubes; Fullerene nanowhiskers; IL-1 beta; NLRP3
资金
- Health, Labor, and Welfare Sciences Research Grant [H21-kagaku-ippan-008, H24-kagaku-shitei-009, H26-kagaku-ippan-004]
- JSPS KAKENHI [23590164]
- Grants-in-Aid for Scientific Research [23590164] Funding Source: KAKEN
Because multi-wall carbon nanotubes (MWCNTs) have asbestos-like shape and size, concerns about their pathogenicity have been raised. Contaminated metals of MWCNTs may also be responsible for their toxicity. In this study, we employed high-temperature calcined fullerene nanowhiskers (HTCFNWs), which are needle-like nanofibers composed of amorphous carbon having similar sizes to MWCNTs but neither metal impurities nor tubular structures, and investigated their ability to induce production a major pro-inflammatory cytokine IL-1 beta via the Nod-like receptor pyrin domain containing 3 (NLRP3)-containing flammasome-mediated mechanism. When exposed to THP-1 macrophages, long-HTCFNW exhibited robust IL-1 beta production as long and needle-like MWCNTs did, but short-HTCFNW caused very small effect. IL-1 beta release induced by long-HTCFNW as well as by long, needle-like MWCNTs was abolished by a caspase-1 inhibitor or siRNA-knockdown of NLRP3, indicating that NLRP3-inflammasome-mediated IL-1 beta production by these carbon nanofibers. Our findings indicate that the needle-like shape and length, but neither metal impurities nor tubular structures of MWCNTs were critical to robust NLRP3 activation. (C) 2014 Elsevier Inc. All rights reserved.
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