期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 5, 期 3, 页码 302-307出版社
ELSEVIER SCI LTD
DOI: 10.1016/S1367-5931(00)00206-4
关键词
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Combinatorial libraries of alanine-substituted proteins can be used to rapidly identify residues important for protein function, stability and shape. Each alanine substitution examines the contribution of an individual amino acid sidechain to the functionality of the protein. The recently described method of shotgun scanning uses phage-displayed libraries of alanine-substituted proteins for high-throughput analysis.
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