期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 441, 期 2, 页码 364-370出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.10.051
关键词
microRNA; Twist1; Chemoresistance; Epithelial-mesenchymal transition; Tongue squamous cell carcinoma
资金
- National Nature Science Foundation of China [NSFC81072228, NSFC81072223, NSFC81272953]
- Guangdong Natural Science Foundation [S2011020002325]
- Ministry of Education [20120171110050]
- fundamental research funds for the Central Universities [11ykzd09]
- program for New Century Excellent Talents in University [NCET-10-0857]
Although many researches have been undertaken to disclose the mechanisms of chemoresistance, the mechanisms remain unclear. The aim of this study is to elucidate the role of miR-181a-Twist1 pathway in the chemoresistance of tongue squamous cell carcinoma (TSCC). We found that cisplatin-induced chemoresistance in TSCC cell lines underwent EMT (epithelial-mesenchymal transition) and was accompanied by enhancing metastatic potential (migration and invasion in vitro), miR-181a downregulation and Twist1 upregulation. Functional analyses indicated that miR-181a reversed chemoresistance, inhibited EMT and metastatic potential in TSCC cells. Twist1 was confirmed as a direct miR-181a target gene by luciferase reporter gene assays. Twist1 knockdown by siRNA led to a reversal of the chemoresistance, inhibited EMT and metastatic potential in TSCC cells. Our study demonstrates that miR-181a-Twist1 pathway may play an important role in the development of cisplatin-chemoresistance, with EMT and an increase the metastatic potential of TSCC cells. (C) 2013 Elsevier Inc. All rights reserved.
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