期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 432, 期 2, 页码 296-301出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.01.113
关键词
Adipocytes; Fat metabolism; Fatty acids; Glycerol; Fat; Lipolysis
资金
- Boston Nutrition and Obesity Research Center (NIH-NIDDK) [P30DK046200]
- Evans Medical Foundation pilot grant
- NIH-NIDDK [R56DK094815, R24 DK087669, DK08257, 5U01ES020958-03]
- USDA Agricultural Service [58-1950-7-707]
Human adipocytes express high levels of two distinct lipid droplet proteins, fat specific protein 27 (FSP27; also called CIDEC), a member of the CIDE family, and perilipin1 (PLIN1), a member of the PAT family. Both proteins play a role in fat metabolism in adipocytes, but how they interact is not known. Our present study demonstrates that FSP27 and PLIN1 co-localize and interact in cultured human primary adipocytes. We also found that the C-terminal domain of FSP27, aa 120-220, interacts with PLIN1. Individual expression of exogenous FSP27 or PLIN1 increased triglyceride content and decreased glycerol release (a measure of lipolysis), but co-expression of both proteins did not further increase triglyceride content or decrease lipolysis in human adipocytes. However, the combination of PLIN1 and FSP27 increased the average size of lipid droplets or caused the formation of unilocular adipocytes. Our data suggest that FSP27 interacts with PLIN1 to regulate lipid droplet size in human adipocytes in a concerted manner. (C) 2013 Elsevier Inc. All rights reserved.
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