期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 437, 期 3, 页码 482-488出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2013.06.111
关键词
Proteomics analysis; Autophagy-deficient MEFs; Atg7; F-actin
资金
- Twelfth Five-Year National Science and Technology Support Program [2012BAI29B06]
- National Natural Science Foundation of China [81274170]
- Jinan University's Scientific Research Creativeness Cultivation Project for Outstanding Undergraduates Recommended for Postgraduate Study
- Major Platform Project Funds of Administration of Ocean and Fisheries of Guangdong, China [GD2012-D01-002]
Autophagy plays a crucial role in a wide array of physiological processes. To uncover the complex regulatory networks and mechanisms underlying basal autophagy, we performed a quantitative proteomics analysis of autophagy-deficient mouse embryonic fibroblast cells (MEFs) using iTRAQ labeling coupled with on-line 2D LC/MS/MS. We quantified a total of 1234 proteins and identified 114 proteins that were significantly altered (90% confidence interval), including 48 up-regulated proteins and 66 down-regulated proteins. We determined that F-actin was disassembled in autophagy-deficient Atg7(-/-) MEFs. Treatment of the WT MEFs with cytochalasin D (CD), which induces F-actin depolymerization, significantly induced autophagosome formation. However, treatment with cytochalasin D also increased the protein level of p62 under starvation conditions, suggesting that depolymerization of F-actin impaired autophagosome maturation and that the intact F-actin network is required for basal and starvation-induced autophagy. Our results demonstrate a close relationship between F-actin and autophagy and provide the basis for further investigation of their interactions. (C) 2013 Elsevier Inc. All rights reserved.
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