期刊
INFECTION AND IMMUNITY
卷 69, 期 6, 页码 4109-4115出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.69.6.4109-4115.2001
关键词
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资金
- NIGMS NIH HHS [T32 GM08347, T32 GM008347] Funding Source: Medline
Pasteurella multocida is the causative agent of a,vide range of diseases in avian and mammalian hosts. Gene expression in response to low iron conditions was analyzed in P. multocida using whole-genome microarrays. The analysis shows that the expression of genes involved in energy metabolism and electron transport generally decreased 2.1- to 6-fold while that of genes used for iron binding and transport increased 2.1- to 7.7-fold in P. multocida during the first 2 h of growth under iron-limiting conditions compared with controls. Notably, 27% of the genes with significantly altered expression had no known function, illustrating the limitations of using publicly available databases to identify genes involved in microbial metabolism and pathogenesis. Taken together, the results of our investigations demonstrate the utility of whole-genome microarray analyses for the identification of genes with altered expression profiles during varying growth conditions and provide a framework for the detailed analysis of the molecular mechanisms of iron acquisition and metabolism in P. multocida and other gram-negative bacteria.
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