期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 431, 期 3, 页码 610-616出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.12.097
关键词
MicroRNA-9 (miR-9); Nasopharyngeal carcinoma; Cytokine; Interferon-inducible gene; MHC class I molecule; Interleukin-related gene
资金
- State Key Development Program for Basic Research of China [2010CB529400]
- Joint Funds of the National Natural Science Foundation of China-Guangdong Province [uo732006]
- National Natural Science Foundation of China [81172587]
- Science and Technology Planning Project of Guangdong Province of China [20098060300008]
- Natural Science Foundation of Guangdong Province of China [S2012010009212]
- Fundamental Research Funds for the Central Universities of China [10ykpy21]
- Science and Technology Talented Man Foundation of Outstanding Young and Middle-aged People of Southern Medical University
- Yat-sen Scholarship for Young Scientists
The functions of miR-9 in some cancers are recently implicated in regulating proliferation, epithelial-mesenchymal transition (EMT), invasion and metastasis, apoptosis, and tumor angiogenesis, etc. miR-9 is commonly down-regulated in nasopharyngeal carcinoma (NPC), but the exact roles of miR-9 dysregulation in the pathogenesis of NPC remains unclear. Therefore, we firstly used miR-9-expressing CNE2 cells to determine the effects of miR-9 overexpression on global gene expression profile by microarray analysis. Microarray-based gene expression data unexpectedly demonstrated a significant number of up-or down-regulated immune- and inflammation-related genes, including many well-known interferon (IFN)-induced genes (e.g., IFI44L, PSMB8, IRF5, PSMB10, IFI27, PSB9_HUMAN, IFIT2, TRAIL, IFITI, PSB8_HUMAN, IRFI, B2M and GBP1), major histocompatibility complex (MHC) class I molecules (e.g., HLA-B, HLA-C, HLA-F and HLA-H) and interleukin (IL)-related genes (e.g., IL2ORB, GALT, IL7, IL1B, ILI I, IL1F8, ILIA, IL6 and IL7R), which was confirmed by qRT-PCR. Moreover, the overexpression of miR-9 with the miRNA mimics significantly up- or down-regulated the expression of above-mentioned IFN-inducible genes, MHC class I molecules and IL-related genes; on the contrary, miR-9 inhibition by anti-miR-9 inhibitor in CNE2 and 5-8F cells correspondingly decreased or increased the aforementioned immune- and inflammation-related genes. Taken together, these findings demonstrate, for the first time, that miR-9 can modulate the expression of IFN-induced genes and MHC class I molecules in human cancer cells, suggesting a novel role of miR-9 in linking inflammation and cancer, which remains to be fully characterized. (C) 2013 Elsevier Inc. All rights reserved.
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