Continuous two-dimensional current source density analyses of electrophysiological activity in hippocampal slices

The well-studied methods of current source density analysis use the Laplacian transform to identify locations and relative magnitudes of current sources and sinks. The method is typically used in reduced one-dimensional form for electrophysiological measures, due to technical limitations. The present paper outlines a two-dimensional method in which simultaneous samples are recorded from multiple electrodes in an equidistant array, enabling the continuous sensing of current flows in any direction within the plane of the slice. This method reveals spatial aspects of the current sources and sinks that are difficult to discern by other means. (C) 2001 Elsevier Science B.V. All rights reserved.