期刊
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
卷 9, 期 2, 页码 107-116出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00022744-200106000-00002
关键词
antigen retrieval; formalin-fixed; paraffin-embedded tissue; immunohistochemistry; in situ hybridization; nucleic acid; test battery; western blotting
资金
- NCI NIH HHS [CA71921] Funding Source: Medline
The impact of the antigen retrieval (AR) technique upon diagnostic immunohistochemistry (IHC) and upon research has been demonstrated by numerous of articles and more than a dozen major reviews. The specific aim of this survey of the field is to examine potential new approaches to retrieval of nucleic acid and protein from archival paraffin-embedded tissue fur molecular biology-based diagnostic procedures that form the basis of the emerging field of molecular morphology. Any new approach must incorporate the principles of standardization and improved reproducibility. The ultimate goal will be to understand the mechanisms of fixation (by formalin) and unfixation (by AR). In the interim, the diligent application of practical procedures that have been shown to be tried and true is the least that we must demand from ourselves and our laboratories.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据