期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 19, 期 2, 页码 129-135出版社
INT SOC PLANT MOLECULAR BIOLOGY
DOI: 10.1007/BF02772155
关键词
aquaporin; epidermis; guard cell; in situ hybridization; Vicia faba
Because the epidermis of V. faba L. leaves easily can be peeled into strips of one cell layer, we developed a simple method of in situ hybridization using epidermal peels as a substitute for paraffin, resin and cryosections. Our method sufficiently detected the expression of broad bean aquaporin 1 in guard cells. RT-PCR revealed higher expression of aquaporins (AQPs) in guard cells compared to other leaf cell types; this indicates the importance of AQP for bulk water flow across guard cell membranes and, therefore, for stomatal movements.
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