期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 419, 期 2, 页码 188-193出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.01.141
关键词
Bioluminescence; Chemiluminescence; Fluorescent protein; Luciferase; Transgenic mouse
资金
- Japanese Ministry of Education, Culture Sports, Science, and Technology (MEXT)
- Funding Program for World-leading Innovative R&D on Science and Technology
- Grants-in-Aid for Scientific Research [22700417, 22390292, 21390062, 23650327] Funding Source: KAKEN
The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an imaging method that uses a chemiluminescent/fluorescent protein, ffLuc-cp156, which consists of a yellow variant of Aequorea GFP and firefly luciferase. We report an improvement in photon yield by over three orders of magnitude over current bioluminescent systems. We imaged cellular movement at high resolution including neuronal growth cones and microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled video-rate bioluminescence imaging of freely moving animals, which may provide a reliable assay for drug distribution in behaving animals for pre-clinical studies. (C) 2012 Elsevier Inc. All rights reserved.
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