期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 426, 期 2, 页码 273-279出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2012.08.086
关键词
GsJAZ2; Functional analysis; Salt and alkali stress tolerance; Marker gene; Glycine soja
资金
- National Natural Science Foundation of China [30570990, 30471059]
- 863 project [2008AA10Z153]
- National Major Project for Cultivation of Transgenic Crops [2008ZX08004]
- Key Research Plan of Heilongjiang Province [GA06B103-3]
- Innovation Research Group of NEAU [CXT004]
- Basic Research Preliminary Study Foundation of the Ministry of Science and Technology of the PRC [2003CCA03500]
Salt and alkali stress are two of the main environmental factors limiting crop production. Recent discoveries show that the JAZ family encodes plant-specific genes involved in jasmonate signaling. However, there is only limited information about this gene family in abiotic stress response, and in wild soybean (Glycine sofa), which is a species noted for its tolerance to alkali and salinity. Here, we isolated and characterized a novel JAZ family gene, GsJAZ2, from G. soja. Transcript abundance of GsJAZ2 increased following exposure to salt, alkali, cold and drought. Over-expression of GsJAZ2 in Arabidopsis resulted in enhanced plant tolerance to salt and alkali stress. The expression levels of some alkali stress response and stress-inducible marker genes were significantly higher in the GsJAZ2 overexpression lines as compared to wild-type plants. Subcellular localization studies using a GFP fusion protein showed that GsJAZ2 was localized to the nucleus. These results suggest that the newly isolated wild soybean GsJAZ2 is a positive regulator of plant salt and alkali stress tolerance. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
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