期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 291, 期 9, 页码 4386-4398出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M115.672931
关键词
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资金
- Center for Molecular and Translational Medicine
- National Natural Science Foundation of China [31440063, 31230047, 31322037, 31271543, 81170538]
- National Basic Research Program of China [2011CB944503, 2011CB944504, 2014CB943200, 2012CB966704, 2011CB966303]
- National Key Technologies Research and Development Program [2012BAI32B01]
- Beijing Municipal Science and Technology Commission [Z131100005213006]
The epigenetic regulation of spatiotemporal gene expression is crucial for human development. Here, we present whole-genome chromatin immunoprecipitation followed by high throughput DNA sequencing (ChIP-seq) analyses of a wide variety of histone markers in the brain, heart, and liver of early human embryos shortly after their formation. We identified 40,181 active enhancers, with a large portion showing tissue-specific and developmental stage-specific patterns, pointing to their roles in controlling the ordered spatiotemporal expression of the developmental genes in early human embryos. Moreover, using sequential ChIP-seq, we showed that all three organs have hundreds to thousands of bivalent domains that are marked by both H3K4me3 and H3K27me3, probably to keep the progenitor cells in these organs ready for immediate differentiation into diverse cell types during subsequent developmental processes. Our work illustrates the potentially critical roles of tissue-specific and developmental stage-specific epigenomes in regulating the spatiotemporal expression of developmental genes during early human embryonic development.
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