期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 276, 期 23, 页码 20735-20742出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M009723200
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资金
- NEI NIH HHS [EY10814] Funding Source: Medline
- NIDDK NIH HHS [DK43812] Funding Source: Medline
- NINDS NIH HHS [NS32578] Funding Source: Medline
Terminal differentiation of lens fiber cells resembles the apoptotic process in that organelles are lost, DNA is fragmented, and changes in membrane morphology occur. However, unlike classically apoptotic cells, which are disintegrated by membrane blebbing and vesiculation, aging lens fiber cells are compressed into the center of the lens, where they undergo cell-cell fusion and the formation of specialized membrane interdigitations. In classically apoptotic cells, caspase cleavage of the cytoskeletal protein alpha -spectrin to similar to 150-kDa fragments is believed to be important for membrane blebbing, We report that caspase(s) cleave alpha -spectrin to similar to 150-kDa fragments and beta -spectrin to similar to 120- and similar to 80-kDa fragments during late embryonic chick lens development. These fragments continue to accumulate with age so that in the oldest fiber cells of the adult lens, most, if not all, of the spectrin is cleaved to discrete fragments. Thus, unlike classical apoptosis, where caspase-cleaved spectrin is short lived, lens fiber cells contain spectrin fragments that appear to be stable for the lifetime of the organism. Moreover, fragmentation of spectrin results in reduced membrane association and thus may lead to permanent remodeling of the membrane skeleton. Partial and specific proteolysis of membrane skeleton components by caspases may be important for age-related membrane changes in the lens.
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