Alzheimer's disease amyloid-β binds copper and zinc to generate an allosterically ordered membrane-penetrating structure containing superoxide dismutase-like subunits

Amyloid beta peptide (A beta) is the major constituent of extracellular plaques and perivascular amyloid deposits, the pathognomonic neuropathological lesions of Alzheimer's disease. Gu(2+) and Zn2+ bind A beta, inducing aggregation and giving rise to reactive oxygen species. These reactions may play a deleterious role in the disease state, because high concentrations of iron, copper, and zinc have been located in amyloid in diseased brains. Here we show that coordination of metal ions to A beta is the same in both aqueous solution and lipid environments, with His(6), His(13), and His(14) all involved. At Cu2+/peptide molar ratios >0.3, A beta coordinated a second Cu2+ atom in a highly cooperative manner. This effect was abolished if the histidine residues were methylated at. N-epsilon2, indicating the presence of bridging histidine residues, as found in the active site of superoxide dismutase, Addition of Cu2+ Or Zn2+ to A beta in a negatively charged lipid environment caused a conformational change from beta -sheet to alpha -helix, accompanied by peptide oligomerization and membrane penetration, These results suggest that metal binding to A beta generated an allosterically ordered membrane-penetrating oligomer linked by superoxide dismutase-like bridging histidine residues.