4.5 Article Proceedings Paper

Estrogen receptor-α is required by the supporting somatic cells for spermatogenesis

期刊

MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 178, 期 1-2, 页码 57-63

出版社

ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0303-7207(01)00410-5

关键词

estrogen receptor; spermatogenesis; germ-cell transplantation; epididymis

向作者/读者索取更多资源

The gene for estrogen receptor-alpha (ER alpha) was disrupted ill embryonic stem cells by homologous recombination and these cells were used to generate mice with a targeted mutation in the ER alpha gene (alpha ERKO mice). It was found that males homozygous for the mutation are infertile, indicating that estrogen signaling through this nuclear hormone receptor is required for male reproductive function. Although spermatogenesis arrears normal in juvenile and young adult alpha ERKO mice, the sperm produced are unable to fertilize eggs in vitro. To determine whether ER alpha is required by somatic or germ cells in the male reproductive tract, we transplanted germ cells from homozygous mutant (ER alpha (-/-)) males to the testes of wild-type (ER alpha (+/+)) males depleted of germ cells by busulfan treatment. The recipients ('surrogate fathers') sired offspring heterozygous for the mutation (ER alpha ('/-) and carrying the coat-color marker of the infertile donor males. This indicated that ER alpha (-/-) germ cells an able to produce sperm competent to fertilize when they are supported by ER alpha (+/+). somatic cells. When ER alpha (+/-) offspring produced by germ cell transplantation were mated to produce ER alpha (-/-) males, these mice were found to have the same phenotype as originally reported for alpha ERKO males. These studies showed that male germ cells do not require ER alpha for regulation of their own genes for development and Function, and strongly imply that somatic cells of the male reproductive tract require ER alpha to support the production of sperm that are capable of fertilization. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据