期刊
EMBO JOURNAL
卷 20, 期 12, 页码 3132-3144出版社
WILEY
DOI: 10.1093/emboj/20.12.3132
关键词
exocytosis; mitochondria; parasitophorous vacuole; rhoptry; trafficking
资金
- NIAID NIH HHS [R01 AI034036, AI 34036] Funding Source: Medline
Rapid discharge of secretory organelles called rhoptries is tightly coupled with host cell entry by the protozoan parasite Toxoplasma gondii, Rhoptry contents were deposited in clusters of vesicles within the host cell cytosol and within the parasitophorous vacuole, To examine the fate of these rhoptry-derived secretory vesicles, we utilized cytochalasin D to prevent invasion, leading to accumulation of protein-rich vesicles in the host cell cytosol. These vesicles lack an internal parasite and are hence termed evacuoles. Like the mature parasite-containing vacuole, evacuoles became intimately associated with host cell mitochondria and endoplasmic reticulum, while remaining completely resistant to fusion with host cell endosomes and lysosomes. In contrast, evacuoles were recruited to pre-existing, parasite-containing vacuoles and were capable of fusing and delivering their contents to these compartments. Our findings indicate that a two-step process involving direct rhoptry secretion into the host cell cytoplasm followed by incorporation into the vacuole generates the parasitophorous vacuole occupied by Toxoplasma. The characteristic properties of the mature vacuole are likely to be determined by this early delivery of rhoptry components.
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