期刊
INTERNATIONAL JOURNAL OF CANCER
卷 92, 期 6, 页码 839-842出版社
WILEY-LISS
DOI: 10.1002/ijc.1270
关键词
beta-catenin; CTNNB1; malignant melanoma; mutation analysis; immunohistochemistry
类别
beta -catenin plays an important role in the Wnt signaling pathway by activating T-cell factor (Tcf)/lymphoid enhancer factor (Lef)-regulated gene transcription. The level of beta -catenin is regulated through GSK-3 beta phosphorylation of specific serine and threonine residues, all of which are encoded for in exon 3 of the beta -catenin gene (CTNNBI), Mutations altering the GSK-3 beta phosphorylation sites lead to cellular accumulation of beta -catenin and constitutive transcription of Tcf/Lef target genes. Such mutations have previously been found in melanoma cell lines. In our study, primary melanomas and their corresponding metastases were screened for CTNNBI exon 3 mutations using single-strand conformation polymorphism and nucleotide sequence analysis. One of 31 primary tumors and 1 of 37 metastases, both originating from the same patient, had a TCT to TTT mutation at codon 45, changing serine to phenylalanine. Immunohistochemical analysis revealed membranous localization of beta -catenin in a majority of the samples, The mutated primary tumor and metastasis, however, displayed widespread cytoplasmic and nuclear expression of beta -catenin, An additional 30% of the primary tumors showed focal cytoplasmic and nuclear staining. Thus, beta -catenin exon 3 mutations are rare in primary as well as metastatic melanomas and do not explain the abnormal cytoplasmic and nuclear localization of beta -catenin round in a relatively large fraction of primary melanomas, (C) 2001 Wiley-Liss. Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据