期刊
JOURNAL OF CHROMATOGRAPHY B
卷 757, 期 2, 页码 301-308出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00163-3
关键词
amide-based stationary phase; thiamine; vitamins
Two reversed-phase chromatographic methods for the separation of thiamine and related compounds are compared. The first procedure is based on the ion-pair technique using an octadecylsilica column, while the second uses a new amide-based stationary phase, which avoids the need to form ion-pairs. leading to narrower peaks and a simpler mobile phase. Analyses were performed by gradient elution and a photo-diode array was used for detection. Specificity was demonstrated by the retention characteristics, UV spectra and by comparing the peak purity index with commercial standards. The procedures were applied to the determination of thiamine-related compounds in pharmaceutical preparations and urine. No preliminary sample treatment was required. (C) 2001 Elsevier Science B.V. All rights reserved.
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