期刊
JOURNAL OF CELL BIOLOGY
卷 153, 期 7, 页码 1465-1478出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.153.7.1465
关键词
nucleoporin; nuclear transport; karyopherin; importin; exportin
类别
资金
- NCRR NIH HHS [RR 00862] Funding Source: Medline
Nucleocytoplasmic transport is mediated by the interplay between soluble transport factors and nucleoporins resident within the nuclear pore complex (NPC). Understanding this process demands knowledge of components of both the soluble and stationary phases and the interface between them, Here, we provide evidence that Nup2p, previously considered to be a typical yeast nucleoporin that binds import- and export-bound karyopherins, dynamically associates with the NPC in a Ran-facilitated manner. When bound to the NPC, Nup2p associates with regions corresponding to the nuclear basket and cytoplasmic fibrils. On the nucleoplasmic face, where the Ran-GTP levels are predicted to be high, Nup2p binds to Nup60p. Deletion of NUP60 kenders Nup2p nucleoplasmic and compromises Nup2p-mediated recycling of Kap60p/Srplp. Depletion of Ran-GTP by metabolic poisoning, disruption of the Ran cycle, or in vitro by cell lysis, results in a shift of Nup2p from the nucleoplasm to the cytoplasmic face of the NPC. This mobility of Nup2p was also detected using heterokaryons where, unlike nucleoporins, Nup2p was observed to move from one nucleus to the other. Together, our data support a model in which Nup2p movement facilitates the transition between the import and export phases of nucleocytoplasmic transport.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据