期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 404, 期 4, 页码 1065-1069出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.12.114
关键词
MicroRNA; Mutation; Strand selection; Microarray; Butterfly effect
资金
- National Science Council, Taiwan [NSC 97-2320-B075-004-MY3]
MicroRNAs (miRNAs) are short nucleotide RNAs that negatively regulate gene expression at the post-transcriptional level. Partially double-stranded miRNAs interact with an RNA-induced silencing complex (RISC) where one strand termed the guide strand is selected, while the partner strand accumulates at a lower level and is presumed to be degraded. The miRNA-loaded RISC then binds to target mRNAs through imperfect complementary sequences located in the 3'UTR and causes translation inhibition. One miRNA may negatively regulate hundreds of target mRNAs. In this study, a pre-miR-155 mutant was used to elucidate that a single mutation creating a mismatch near the 3' end of miR-155 led to a shift in strand selection, causing an increased selection of miR-155(*) and a decreased selection of miR-155, thereby fine-tuning the translation of their target genes. Consequently, this resulted in a butterfly effect on global gene expression. Indeed, nearly half of the genes we analyzed in this study showed altered expression. Provided that over 800 miRNAs have been identified in humans to date, mutation of miRNA is expected to play a critical role in species evolution and individual diversity. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据