期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 412, 期 1, 页码 136-142出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2011.07.059
关键词
Glycosaminoglycans; Heparan sulfate; Dengue virus; Japanese encephalitis virus; Anticoagulant activity
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [21590057, 23390016]
- Research Center for Zoonosis Control
- Hokkaido University
- Grants-in-Aid for Scientific Research [21590057, 23390016] Funding Source: KAKEN
The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications. (C) 2011 Elsevier Inc. All rights reserved.
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