期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 404, 期 1, 页码 297-301出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.11.110
关键词
PD153035; Non-small cell lung cancer; Tyrosine kinase inhibitor; Binding affinity constant; Optical tweezers; Wormlike chain model
资金
- Taipei Medical University [TMU96-AE1-B36]
- Cathay General Hospital/Taipei Medical University [97CGH-TMU-15]
- Taipei Medical University/Taipei Medical University Hospital [98TMU-TMUH-03-3, 99TMU-TMUH-03-4]
Accurately predicting binding affinity constant (K-A) is highly required to determine the binding energetics of the driving forces in drug-DNA interactions. Recently, PD153035, brominated anilinoquinazoline, has been reported to be not only a highly selective inhibitor of epidermal growth factor receptor but also a DNA intercalator. Here, we use a dual-trap optical tweezers to determining K-A for PD153035, where K-A is determined from the changes in B-form contour length (L) of PD153035-DNA complex. Here, L is fitted using a modified wormlike chain model. We found that a noticeable increment in L in 1 mM sodium cacodylate was exhibited. Furthermore, our results showed that K-A = 1.18(+/-0.09) x 10(4) (1/M) at 23 +/- 0.5 degrees C and the minimum distance between adjacent bound PD153035 approximate to 11 bp. We anticipate that by using this approach we can determine the complete thermodynamic profiles due to the presence of DNA intercalators. (C) 2010 Elsevier Inc. All rights reserved.
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