期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 400, 期 3, 页码 293-298出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.08.023
关键词
Toxin-antitoxin system; Rv1102c; Rv1103c; Mycobacterium tuberculosis
资金
- Wayne State University
- Marine & Extreme Genome Research Center Program (KORDI, Republic of Korea)
- 21C Frontier Microbial Genonnics and Application Center program (Republic of Korea)
- BK21 Project (Brain-Korea 21)
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20046001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [11-2008-16-001-00, 과C6A2205] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Toxin-antitoxin systems, ubiquitous in piokaryotic genomes, have been proposed to play an important role in several stress responses. While Mycobacterium tuberculosis contains more than 80 putative TA loci, the roles they play in this pathogen are yet to be studied Here, we characterize a chromosomal Rv1102c-Rv1103c TA system in M tuberculosis. We found that the Rv1102c toxin interacts with the Rv1103c antitoxin in a pull-down assay and the yeast two-hybrid system Rv1102c cleaved the era mRNA in Escherichia colt, and cleavage was inhibited by co-expression of Rv1103c Heterologous expression of Rv1102c led to growth arrest in E coli, which was fully recovered only when Rv1103c was co-expressed in cis with Rv1102c, suggesting that the production and assembly of Rv1102c and Rv1103c are tightly linked. Our additional results indicate that translational coupling of the Rv1102c and Rv1103c genes is important for Rv1102c-Rv1103c binding. Finally, we discovered that the expression of Rv1102c induced growth arrest and Increased the level of persister cells in Mycobacterium smegmatis These results suggest that the Rv1102c-Rv1103c TA system could play a role in M tuberculosis pathogenesis via generating bacilli that survive in the face of multidrug therapy (C) 2010 Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据