期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 395, 期 3, 页码 318-323出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.03.173
关键词
Rab27; Coronin; Endocytosis; Insulin granule; Pancreatic beta-cell; Small GTPase
资金
- KAKENHI [19790636, 21790876]
- Takeda Science Foundation
- Discretion of the President, Oita University
- Grants-in-Aid for Scientific Research [19790636, 21790876] Funding Source: KAKEN
GTP-Rab27a is known to regulate insulin exocytosis. We have recently reported that coronin 3, which paradoxically binds GDP-Rab27a, participates in endocytosis of the insulin secretory membrane. Here, we demonstrate that glucose stimulation caused redistribution of coronin 3 in the vicinity of the plasma membrane, which was mimicked by overexpression of the GDP-Rab27a mutant or the Rab27a GAP. Glucose-induced translocation of coronin 3 was inhibited by Rab27a knock-down. The internalized phogrin, an insulin granule associated protein, located near the plasma membrane by the dominant-negative coronin 3, but the protein at the outer surface of the plasma membrane was decreased. These results indicate that glucose recruits coronin 3 near the plasma membrane, and that it regulates the retrograde transport of the secretory membrane in pancreatic beta-cells. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据