4.6 Article

Real-time visualization of prion transport in single live cells using quantum dots

期刊

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.02.159

关键词

Prion; Quantum dot; Visualization; Live cell; Lipid raft

资金

  1. National Key Scientific Program Nanoscience and Nanotechnology [2006CB933100]
  2. National Natural Science Foundation of China [30970150, 20921062, 20833006]
  3. Program for Changjiang Scholars and Innovative Research Team in University [IRT0745]

向作者/读者索取更多资源

Prion diseases ale fatal neurodegenerative disorders resulting from structural conversion of the cellular isoform of PrPC to the infectious scrapie isoform PrPSc. It is believed that such structural alteration may occur within the internalization pathway However, there is no direct evidence to support this hypothesis Employing quantum dots (QDs) as a probe, we have recorded a real-time movie demonstrating the process of prion internalization in a living cell for the first time The entire internalization process can be divided into four discrete but connected stages In addition, using methyl-beta-cyclodextrin to disrupt cell membrane cholesterol, we show that lipid rafts play an important role in locating cellular PrPC to the cell membrane and in initiating PrPC endocytosis (C) 2010 Elsevier Inc All rights reserved

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