期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 21, 期 13, 页码 4311-4320出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.13.4311-4320.2001
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资金
- NIGMS NIH HHS [R01 GM056700, GM56700] Funding Source: Medline
Snf-Swi, the prototypical ATP-dependent nucleosome-remodeling complex, regulates transcription of a subset of yeast genes. With the exception of Snf2p, the ATPase subunit, the functions of the other components are unknown. We have investigated the role of the conserved Snf-Swi core subunit Snf5p through characterization of two conditional snf5 mutants. The mutants contain single amino acid alterations of invariant or conserved residues that abolish Snf-Swi-dependent transcription by distinct mechanisms. One mutation impairs Snf-Swi assembly and, consequently, its stable association with a target promoter. The other blocks a postrecruitment catalytic remodeling step. These findings suggest that Snf5p coordinates the assembly and nucleosome-remodeling activities of Snf-Swi.
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